ADS BIOTEC Taq DNA Polymerase, 4 x 250 Units (5 Units /µL). Supplied with both Mg2+ containing buffer and Mg2+ free buffer and separate Mg2+ solution (2 x 1.5 mL of each)
T-Taq DNA Polymerase (1000 U)
ADS BIOTEC Taq DNA polymerase (T-Taq) is a recombinant, thermostable, 94-kDa DNA polymerase encoded in Escherichia coli by a modified form of a DNA polymerase gene from Thermus aquaticus. The enzyme possess 5’->3’ DNA polymerase activity and 5’->3’ DNA exonuclease activity, but not 3’->5’ DNA exonuclease activity. ADS BIOTEC Taq DNA polymerase is > 95% homogeneous by SDS-PAGE and lacks detectable DNA endonuclease, DNA exonuclease, DNA nicking activity, and DNA priming activity. The enzyme generates PCR products with 3’-dA overhangs. Each lot is prequalified for use in PCR amplification.
Reaction mixtures (25 µl) contained 1X Reaction Buffer recommended by the manufacturer, 200 µM of each dNTP, 50 ng human genomic DNA, 4 pmoles (50 ng) each of forward and reverse primer for various genes, and 1.25 units of Taq or AmpliTaq DNA polymerase. After a 2 min denaturation step at 94°C, 30 cycles of amplification were performed composed of 94°C, 30 sec; 60°C, 30 sec; and 72°C for 1 to 4 min depending upon amplicon length. DNA products were analyzed on a 1% D1-LE agarose gel. Products amplified from several genes are as follows: p53 – lanes 1-4; Lanes 5 and 6 – MSH52; Lanes 7 and 8 – TFRC; Lanes 9 and 10 – RBBP7; Lanes 11 and 12 – IGF2R. Amplicons were p53 (913 bp), p53 (1,587 bp), MSH5 (2,000 bp), TFRC (2,700 bp), and RBBP7 (3,000 bp). Lanes 1, 3, 5, 7, 9, and 11 contain product synthesized by AmpliTaq DNA polymerase. Lanes 2, 4, 6, 8, 10, and 12 contain product synthesized by ADS BIOTEC Taq DNA Polymerase. Lane M contains 1 Kb Plus DNA Ladder (Invitrogen).
- General PCR
- Differential Display
- PCR-based fingerprinting (VNTR, STR, and RAPD)