HPLC Nucleic Acid Separation and Purification

HPLC Nucleic Acid Separation and Purification 2018-07-25T13:26:23+00:00

HPLC Columns and Buffers for Nucleic Acid Separations

ADS Biotec offers first-in-class columns and buffers for the separation and purification of RNA and DNA molecules. The RNASep™ and DNASep™ line of columns are comprised of uniform alkylated nonporous or macro-porous polystyrene-divinylbenzene (PS-DVB) copolymer) microspheres, providing high performance nucleic acid separations. These versatile columns can be used with most HPLC systems for many RNA\DNA separations and purification.

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Optimized HPLC Buffers

Truncated oligonucleotides and other impurities (e.g. deprotection failures) must be removed for most molecular biology applications. This is to ensure their efficacy for downstream application and possible therapeutic use.  Separation and purification of nucleic acids is most commonly performed using ion-paired reversed phase chromatography2,3.  This technique provides ionic interaction between the nucleic acid and the stationary phase via its hydrophobic moiety.

ADS Biotec has more than 20 years’ experience producing the highest quality HPLC buffers available, built upon the solid foundation of our flagship WAVE HPLC system.  ADS Biotec offers 2 types of ion-pair buffers, one using triethylammonium acetate (TEAA) suitable for common nucleic acid separations and the second made using hexylammonium acetate (HAA) for challenging separations.  Both ion-pairing reagents undergo quality testing prior to buffer manufacture to ensure clean baselines, optimal resolution, and separation consistency.   Our hexylammonium acetate (HAA) buffers were developed for superior resolution of sequence independent molecules; capable of resolving molecules of identical length but differing in sequence (weight).

Custom Buffer Manufacture

Additionally, ADS BIOTEC provides custom mobile phase manufacturing of “ready to use” buffer solutions within our proven ISO 9001 Quality System. Depending on your specific application needs, with mixing capability for aqueous and non-aqueous solutions from single liters to a thousand liters, custom labeled and packaged to meet your requirements, ADS BIOTEC can provide storage and global distribution of custom mobile phases. Our flexible manufacturing facility is equipped with dedicated mixing tanks and calibrated equipment all housed in a HEPA-filtered clean environment.