The HANABI-PV Metaphase Auto-Spreader and the HANABI-PVI Metaphase Spreader are instruments that provide optimized environmental conditions for the preparation of metaphase chromosome spreads. The HANABI-PV automatically processes up to 10 samples and prepares up to 20 slides per run. Temperature, humidity and airflow are set and rapidly stabilize, thus ensuring that when samples are dropped and chromosomes spread on microscope slides, they are dried in a consistent and reproducible manner. Excessive spreading and chromosome overlaps are minimized. The sample tubes and the slides are traceable by bar codes. After automated spreading , the spreads on the slides can be further treated and stained to be analyzed.
Chromosome Spreading
- Description
- Overview
- HANABI PV Video
- Specifications
- Support and Warranty
- Order
- Application Notes and Posters
- Product Documents
Product Description
ADS BIOTEC offers two spreader platforms to meet the requirements of small to large sample throughput laboratories
Chromosome Spreading with the HANABI-PV Metaphase Auto-Spreader and the HANABI-PVI Metaphase Spreader provide optimized environmental conditions for the preparation of metaphase chromosome spreads. The HANABI-PV automatically processes up to 10 samples and prepares up to 20 slides per run. Temperature, humidity and airflow are set and rapidly stabilize, thus ensuring that when samples are dropped and chromosomes spread on microscope slides, they are dried in a consistent and reproducible manner. Excessive spreading and chromosome overlaps are minimized. The sample tubes and the slides are traceable by bar codes. After automated spreading , the spreads on the slides can be further treated and stained to be analyzed.
The chromosome spreading process for conventional FISH, CGH and G-banding analysis of samples cultured and harvested from blood, bone marrow, and similar fluids is now streamlined and is easily carried out regardless of skill level.
The HANABI-PV Metaphase Auto-Spreader is an automated instrument that provides optimized environmental conditions for the preparation of metaphase chromosome spreads. The HANABI-PV automatically processes up to 10 samples and prepares up to 20 slides per run. Temperature, humidity and airflow are set and rapidly stabilize, thus ensuring that when samples are dropped and chromosomes spread on microscope slides, they are dried in a consistent and reproducible manner. Excessive spreading and chromosome overlaps are minimized. The sample tubes and the slides are trackable by bar codes. After automated spreading , the spreads on the slides can be further treated and stained to be analyzed.
The chromosome spreading process for conventional FISH, CGH and G-banding analysis of samples cultured and harvested from peripheral blood, bone marrow, and similar fluids is now streamlined and is easily carried out regardless of skill level.
ADS BIOTEC offers three spreader platforms to meet the requirements of small to large sample throughput laboratories.
| HANABI PVI | Manual sample dropping in controlled humidity chamber |
| HANABI PV | Automated, sample throughput from 1 to 10 tubes and up to 20 slides |
| Item | PV Specifications | Remarks |
|---|---|---|
| Number of Samples | 10 samples | Uses bar code reader |
| Number of Slides | 20 slides | Uses bar code reader |
| Size of Sample Tube | 15 mL conical | D=17 mm, PP, PS |
| Slide Glass | 76.5 x 26 (t+0.8-1.2) mm | 76 x 26, 75 x 25 |
| Dispenser Tip | Gilson D200 | Uses 200 µL tip |
| Sample Volume | 0.2 - 4.0 mL | Min: 0.2 mL, Effective amount: 1.0 mL |
| Dropped Volume of Sample | 5 - 40 µL/spot | 2 spots mode: 5 - 30 µL/spot |
| Dropped Volume of Fixative | 5 - 40 µL/spot | 2 spots mode: 5 - 40 µL/spot |
| Number of Spot(s) per slide | 1 - 2 | |
| Additional Fixative Dropping Time | 30 - 90 sec | Using Fixative-adding mode |
| Fully Loaded Processing Time | 1.0 hrs (approx.) | 48 samples, 96 slides |
| Tilting Base | 0/5/10/15 degrees | Using Tilting mode (PIV) / Tilting guide(PV) |
| Dry-Index Settings | 6 – 15 | HANABI dry-index allows drying optimization |
| Bar Code Reader | 30 digits (max) [optional] | QR, DataMatrix, RSS, Barcode |
| Operating Environment | 15 – 25 ºC, 20 – 60% RH | Non-condensing, |
| Storage Environment | 0 – 40 ºC, 9 –95% RH | Non-condensing, non-icing |
| Atmospheric Pressure | 800 – 114 hPa | 2000 meter or lower |
| Power Input Voltage | 100, 120, 230VAC | 50/60 Hz |
| Overvoltage Category | 2 | |
| External Size | 700 W x 800 H x 600 D | Without door knob and switches |
| Weight | 65 Kg (approx.) |
Support Plan
Click here for the pdf version of the PVI support plan.
Click here for the pdf version of the PV support plan.
ADS BIOTEC realizes the importance of maintaining your laboratory’s smooth and efficient operation. The HANABI-PV Metaphase Auto-Spreader requires appropriate care and maintenance for optimal performance. Planning post-warranty maintenance is a critical part of laboratory management. Through the HANABI-PV Metaphase Auto-Spreader Support Plans, we take care of all your post-warranty service needs. This plan includes an annual preventative maintenance program and emergency service support to minimize unscheduled downtime for repairs.
| Catalog Number | Name | Description |
|---|---|---|
| HCMS-99-0001-A | HCMS-99-0001-A HANABI-PVI Manual Spreader | Manual instrument that provides optimized environmental conditions for the preparation of metaphase chromosome spreads. |
| HCMS-99-0002-A | HANABI-PVI Manual Spreader, Tilt Model | Manual instrument that provides optimized environmental conditions for the preparation of metaphase chromosome spreads. Includes Slide Tilt Kit for larger coverage of cell suspension on glass slides. |
| HCMS-99-0005-A | HANABI PV METAPHASE SPREADER | Fully automated instrument that provides optimized environmental conditions for the preparation of metaphase chromosome spreads. The HANABI-PV automatically processes up to 10 samples and up to 20 slides per run. |
| SER-SPRE PV | HANABI-PV Metaphase Auto-Spreader Support Plan | One year instrument service support plan including one preventative maintenance visit. |
