Chromosome Spreading

The HANABI-PV Metaphase Auto-Spreader and the HANABI-PVI Metaphase Spreader are instruments that provide optimized environmental conditions for the preparation of metaphase chromosome spreads. The HANABI-PV  automatically processes up to 10 samples and prepares up to 20 slides per run. Temperature, humidity and airflow are set and rapidly stabilize, thus ensuring that when samples are dropped and chromosomes spread on microscope slides, they are dried in a consistent and reproducible manner.   Excessive spreading and chromosome overlaps are minimized. The sample tubes and the slides are traceable by bar codes. After automated spreading , the spreads on the slides can be further treated and stained to be analyzed.

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Product Description

ADS BIOTEC  offers two spreader platforms to meet the requirements of small to large sample throughput laboratories

Chromosome Spreading with the HANABI-PV Metaphase Auto-Spreader and the HANABI-PVI Metaphase Spreader provide optimized environmental conditions for the preparation of metaphase chromosome spreads. The HANABI-PV  automatically processes up to 10 samples and prepares up to 20 slides per run. Temperature, humidity and airflow are set and rapidly stabilize, thus ensuring that when samples are dropped and chromosomes spread on microscope slides, they are dried in a consistent and reproducible manner.   Excessive spreading and chromosome overlaps are minimized. The sample tubes and the slides are traceable by bar codes. After automated spreading , the spreads on the slides can be further treated and stained to be analyzed.

The chromosome spreading process for conventional FISH, CGH and G-banding analysis of samples cultured and harvested from  blood, bone marrow, and similar fluids is now streamlined and  is easily carried out regardless of skill level.

The HANABI-PV Metaphase Auto-Spreader is an automated instrument that provides optimized environmental conditions for the preparation of metaphase chromosome spreads. The HANABI-PV  automatically processes up to 10 samples and prepares up to 20 slides per run. Temperature, humidity and airflow are set and rapidly stabilize, thus ensuring that when samples are dropped and chromosomes spread on microscope slides, they are dried in a consistent and reproducible manner.   Excessive spreading and chromosome overlaps are minimized. The sample tubes and the slides are trackable by bar codes. After automated spreading , the spreads on the slides can be further treated and stained to be analyzed.

hanabiThe chromosome spreading process for conventional FISH, CGH and G-banding analysis of samples cultured and harvested from peripheral blood, bone marrow, and similar fluids is now streamlined and  is easily carried out regardless of skill level.

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ADS BIOTEC offers three spreader platforms to meet the requirements of small to large sample throughput laboratories.

HANABI PVIManual sample dropping in controlled humidity chamber
HANABI PVAutomated, sample throughput from 1 to 10 tubes and up to 20 slides

HANABI PV Video

ItemPV
Specifications
Remarks
Number of Samples10 samplesUses bar code reader
Number of Slides20 slidesUses bar code reader
Size of Sample Tube15 mL conicalD=17 mm, PP, PS
Slide Glass76.5 x 26 (t+0.8-1.2) mm76 x 26, 75 x 25
Dispenser TipGilson D200Uses 200 µL tip
Sample Volume0.2 - 4.0 mLMin: 0.2 mL, Effective amount: 1.0 mL
Dropped Volume of Sample5 - 40 µL/spot2 spots mode: 5 - 30 µL/spot
Dropped Volume of Fixative5 - 40 µL/spot2 spots mode: 5 - 40 µL/spot
Number of Spot(s) per slide1 - 2 
Additional Fixative Dropping Time30 - 90 secUsing Fixative-adding mode
Fully Loaded  Processing Time1.0 hrs (approx.)48 samples, 96 slides
Tilting Base0/5/10/15 degreesUsing Tilting mode (PIV) / Tilting guide(PV)
Dry-Index Settings6 – 15HANABI dry-index allows drying optimization
Bar Code Reader30 digits (max)
[optional]
QR, DataMatrix, RSS, Barcode
Operating Environment15 – 25 ºC,
20 – 60% RH
Non-condensing,
Storage Environment0 – 40 ºC,
9 –95% RH
Non-condensing, non-icing
Atmospheric Pressure800 – 114 hPa2000 meter or lower
Power Input Voltage100, 120, 230VAC50/60 Hz
Overvoltage Category2 
External Size700 W x 800 H x 600 DWithout door knob and switches
Weight65 Kg (approx.) 

Support Plan

Click here for the pdf version of the PVI support plan.

Click here for the pdf version of the PV support plan.

 

ADS BIOTEC realizes the importance of maintaining your laboratory’s smooth and efficient operation. The HANABI-PV Metaphase Auto-Spreader requires appropriate care and maintenance for optimal performance. Planning post-warranty maintenance is a critical part of laboratory management. Through the HANABI-PV Metaphase Auto-Spreader Support Plans, we take care of all your post-warranty service needs. This plan includes an annual preventative maintenance program and emergency service support to minimize unscheduled downtime for repairs.

Catalog Number Name Description
HCMS-99-0001-A HCMS-99-0001-A HANABI-PVI Manual Spreader Manual instrument that provides optimized environmental conditions for the preparation of metaphase chromosome spreads.
HCMS-99-0002-A HANABI-PVI Manual Spreader, Tilt Model Manual instrument that provides optimized environmental conditions for the preparation of metaphase chromosome spreads. Includes Slide Tilt Kit for larger coverage of cell suspension on glass slides.
HCMS-99-0005-A HANABI PV METAPHASE SPREADER Fully automated instrument that provides optimized environmental conditions for the preparation of metaphase chromosome spreads. The HANABI-PV automatically processes up to 10 samples and up to 20 slides per run.
SER-SPRE PV HANABI-PV Metaphase Auto-Spreader Support Plan One year instrument service support plan including one preventative maintenance visit.

HANABI PV Poster

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