Optimized buffers were specifically developed to provide highly reproducible retention times with HPLC System instrumentation. Optimized buffers have a longer shelf life than buffers prepared from concentrate. They will allow you to generate reproducible data from day to day, week to week, system to system and lab to lab. Not only will they save you time, but they will also reduce problems with water quality and maintenance of purification systems.
Optimized buffers are made in dedicated tanks under scrupulously controlled conditions to ensure clean baselines and long shelf life. For accuracy and consistency, all measurements are performed gravimetrically rather than volumetrically. Most alternative buffers are made in small batches, which typically leads to variations. For example, foreign particles in the volumetric flask can contaminate the buffer, evaporation can affect the concentration of acetonitrile, and fluctuations in temperature can change the volume of the buffer. The possibility of contamination is also higher from non-certified sources of de-ionized water. Optimized Buffers are designed and prepared with optimal reagents and conditions to eliminate these problems.
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Figure 1. Repetitive Low-Range Mutation Standard Injections 10 µL
One of the advantages of Optimized buffers is stability. Because the buffers are manufactured under an inert atmosphere, they are stable during storage and after the bottles are opened. From the first to last drop of Optimized Buffer A and B, retention of the heteroduplexes and resolution of the homoduplexes were reproducible for 500 consecutive injections.
Figure 2. Repetitive DNA Sizing Control Sample Injections 10 μL
Retention times are consistent throughout this series of injections; corresponding peak heights and baseline resolution are unchanged.