How are organoids cultured?
Typically, organoids are cultured within BME domes or layers in multi-well plates surrounded or layered with culture media. The three-dimensional culture in BME more closely mimics the three-dimensional microenvironment found within tissues. BME is porous enough that culture media, growth factors, and signaling factors can diffuse to the cell clusters, and waste can diffuse away from the cell clusters. Tissue specific medias are used so that factors normally found in the tissue microenvironment are present in the culture media to promote growth.