DNASep™ Cartridge – DNA Separation Column

4.6 mm x 50 mm – For high resolution HPLC ds/ss DNA analysis, sizing, and Mutation Detection. Load capacity of 2µg.

SKU: DNA-99-3510 Categories: ,

Description

The DNASep– DNA separation column uses alkylated non-porous polystyrene-divinylbenzene (PS-DVB) copolymer microspheres for high performance nucleic acid separations. These versatile columns can be used with the WAVE­® and a variety of other HPLC Systems.

DNASep ColumnDNA Sizing

  • DNA\Oligo purity assessment
  • Excellent sensitivity and resolution
  • Superior DNA sizing
  • Robust, long-lifetime
  • Hundreds of literature references with WAVE™ dHPLC system

Item Number: DNA-99-3510

4.6 x 50 mm, non-porous PS\DVB resin matrix, column capacity 2.0 μg total DNA.

DNA Sizing

Liquid chromatography (LC) is a powerful technique for separation and quantitation of nucleic acids due to its high-resolving capability, short analysis time, and ease of recovery of DNA fragments for subsequent studies. Using the DNASep™ column for sizing applications in reversed-phase HPLC provides many advantages. Fragments can be collected and subjected to further molecular analysis such as target verification, sub cloning, amplification and sequencing.   DNA fragment sizing is required for quality control (QC) of sequencing templates; quantitation and sizing of DNA fragments; “yes/no” assays for the presence of a DNA fragment in genotyping. Sizing of dsDNA fragments by ion-pair reversed-phase HPLC is largely independent of fragment sequence and sequence related secondary structures of the molecule.

Documents

  • Application Note: Sizing of DNA Fragments with the WAVE® Nucleic Acid Fragment Analysis System
  • Application Note: QC and Purification of Oligos on the WAVE
  • Application Note: DNASep and DNASep HT Cartridge User Guide and Warranty
  • Buffers


    ThumbnailsSKUProducts
    554306
    DNASep® Wash Solution
    4 mL bottle of DNASep® Wash Solution
    553424 - Hexylammonium Acetate Buffer A
    553424
    Hexylammonium Acetate Buffer A – 0.1 M HAA in 10% Acetonitrile
    Case of 4 x 2.5 liter bottles - Hexylammonium Acetate Buffer A
    553425 - Hexylammonium Acetate Buffer B
    553425
    Hexylammonium Acetate Buffer B – 0.1 M HAA in 50% Acetonitrile
    Case of 4 x 2.5 liter bottles - Hexylammonium Acetate Buffer B
    553423 - HPLC Column Wash Solution D
    553423
    HPLC Column Wash Solution D – 75% Acetonitrile
    Case of 4 x 2.5 liter bottles - HPLC Column Wash Solution D
    553421 - Triethylammonium Acetate Buffer A
    553421
    Triethylammonium Acetate Buffer A – 0.1 M TEAA in Water
    Case of 4 x 2.5 liter bottles - Triethylammonium Acetate Buffer A
    553422 - Triethylammonium Acetate Buffer B
    553422
    Triethylammonium Acetate Buffer B – 0.1 M TEAA in 25% Acetonitrile
    Case of 4 x 2.5 liter bottles - Triethylammonium Acetate Buffer B
    SP5890 Triethylammonium Acetate Solution
    SP5890
    Triethylammonium Acetate Solution (TEAA) 2.0 M
    Case of 6 x 200 mL bottles - Triethylammonium Acetate solution 2.0 M
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